MersV1, Main, Exploration, bibRecord, 003590

Characterisation of a protective linear B cell epitope against feline parvoviruses

Identifieur interne : 003590 ( Main/Exploration ); précédent : 003589; suivant : 003591

Characterisation of a protective linear B cell epitope against feline parvoviruses

Auteurs : Jan P. M. Langeveld [Pays-Bas] ; Jorge Martinez-Torrecuadrada [Espagne] ; Ronald S. Boshuizen [Pays-Bas] ; Rob H. Meloen [Pays-Bas] ; J. Ignacio Casal [Espagne]

Source :

Vaccine [ 0264-410X ] ; 2001.

RBID : ISTEX:2E63C5A7E13423A3CB64ADD4311E0C1C1F5DCF68

English descriptors

Abstract

Abstract: Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and the contribution of each residue to the binding, using multiple length analysis. Moreover, a replacement analysis allowed determining those critical residues for the binding. Finally, in an attempt to optimise the production cost of the vaccine, we have determined that the minimal dose required for induction of protective antibodies can be as low as 0.5 μg of peptide. Also, KLH can be replaced as a carrier for a much cheaper alternative such as ovalbumine. All these findings implicate a substantial reduction in the cost of the vaccinal dose.

Url:

https://api.istex.fr/ark:/67375/6H6-RLN9657K-C/fulltext.pdf

DOI: 10.1016/S0264-410X(00)00526-0

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001664
to stream Istex, to step Curation: 001664
to stream Istex, to step Checkpoint: 000F12
to stream Main, to step Merge: 003628
to stream Main, to step Curation: 003590

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterisation of a protective linear B cell epitope against feline parvoviruses</title>
<author><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name>
</author>
<author><name sortKey="Martinez Torrecuadrada, Jorge" sort="Martinez Torrecuadrada, Jorge" uniqKey="Martinez Torrecuadrada J" first="Jorge" last="Martinez-Torrecuadrada">Jorge Martinez-Torrecuadrada</name>
</author>
<author><name sortKey="Boshuizen, Ronald S" sort="Boshuizen, Ronald S" uniqKey="Boshuizen R" first="Ronald S." last="Boshuizen">Ronald S. Boshuizen</name>
</author>
<author><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name>
</author>
<author><name sortKey="Ignacio Casal, J" sort="Ignacio Casal, J" uniqKey="Ignacio Casal J" first="J." last="Ignacio Casal">J. Ignacio Casal</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:2E63C5A7E13423A3CB64ADD4311E0C1C1F5DCF68</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1016/S0264-410X(00)00526-0</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-RLN9657K-C/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001664</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001664</idno>
<idno type="wicri:Area/Istex/Curation">001664</idno>
<idno type="wicri:Area/Istex/Checkpoint">000F12</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F12</idno>
<idno type="wicri:doubleKey">0264-410X:2001:Langeveld J:characterisation:of:a</idno>
<idno type="wicri:Area/Main/Merge">003628</idno>
<idno type="wicri:Area/Main/Curation">003590</idno>
<idno type="wicri:Area/Main/Exploration">003590</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Characterisation of a protective linear B cell epitope against feline parvoviruses</title>
<author><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>ID-Lelystad, Edelhertweg 13, Lelystad</wicri:regionArea>
<wicri:noRegion>Lelystad</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Martinez Torrecuadrada, Jorge" sort="Martinez Torrecuadrada, Jorge" uniqKey="Martinez Torrecuadrada J" first="Jorge" last="Martinez-Torrecuadrada">Jorge Martinez-Torrecuadrada</name>
<affiliation wicri:level="2"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>INGENASA, Hnos Garcia Noblejas 41, 28037 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Boshuizen, Ronald S" sort="Boshuizen, Ronald S" uniqKey="Boshuizen R" first="Ronald S." last="Boshuizen">Ronald S. Boshuizen</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>ID-Lelystad, Edelhertweg 13, Lelystad</wicri:regionArea>
<wicri:noRegion>Lelystad</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>ID-Lelystad, Edelhertweg 13, Lelystad</wicri:regionArea>
<wicri:noRegion>Lelystad</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ignacio Casal, J" sort="Ignacio Casal, J" uniqKey="Ignacio Casal J" first="J." last="Ignacio Casal">J. Ignacio Casal</name>
<affiliation wicri:level="1"><country wicri:rule="url">Espagne</country>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>INGENASA, Hnos Garcia Noblejas 41, 28037 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Vaccine</title>
<title level="j" type="abbrev">JVAC</title>
<idno type="ISSN">0264-410X</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="2001">2001</date>
<biblScope unit="volume">19</biblScope>
<biblScope unit="issue">17–19</biblScope>
<biblScope unit="page" from="2352">2352</biblScope>
<biblScope unit="page" to="2360">2360</biblScope>
</imprint>
<idno type="ISSN">0264-410X</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0264-410X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Canine parvovirus</term>
<term>Monoclonal antibodies</term>
<term>Peptide vaccine</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acid level</term>
<term>Aluminium hydroxide</term>
<term>Amino</term>
<term>Amino acid</term>
<term>Amino acids</term>
<term>Amino terminus</term>
<term>Antibody binding</term>
<term>Antibody titres</term>
<term>Antigenic</term>
<term>Antigenic site</term>
<term>Antigenic sites</term>
<term>Antipeptide</term>
<term>Antipeptide antibodies</term>
<term>Canine</term>
<term>Canine parvovirus</term>
<term>Casal</term>
<term>Coating antigen</term>
<term>Core sequence</term>
<term>Deletion scan</term>
<term>Elisa</term>
<term>Elsevier science</term>
<term>Epitope</term>
<term>Feline parvoviruses</term>
<term>Guinea pigs</term>
<term>Guinea pigs immunised</term>
<term>High dilution</term>
<term>Horse serum</term>
<term>Ignacio casal</term>
<term>Immunised</term>
<term>Lange6eld</term>
<term>Linear epitope</term>
<term>Linear epitopes</term>
<term>Maternal immunity</term>
<term>Minimal amount</term>
<term>Minimal dose</term>
<term>Minimal sequence</term>
<term>Monoclonal antibodies</term>
<term>Natural host</term>
<term>Negative effect</term>
<term>Neutralisation</term>
<term>Neutralising</term>
<term>Neutralising activity</term>
<term>Neutralising antibodies</term>
<term>Neutralising capability</term>
<term>Neutralising mabs</term>
<term>Neutralising titres</term>
<term>Nucleotide sequence</term>
<term>Parvovirus</term>
<term>Pepscan</term>
<term>Pepscan analysis</term>
<term>Peptide</term>
<term>Peptide qpdggqpavr</term>
<term>Peptide synthesis</term>
<term>Peptide vaccine</term>
<term>Rabbits immunised</term>
<term>Replacement analysis</term>
<term>Sequence gqpavrnera</term>
<term>Synthetic peptide</term>
<term>Synthetic peptides</term>
<term>Target animals</term>
<term>Terminus</term>
<term>Titre</term>
<term>Vaccine</term>
<term>Vaccine formulation</term>
<term>Viral disease</term>
<term>Virol</term>
<term>Virus neutralisation</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and the contribution of each residue to the binding, using multiple length analysis. Moreover, a replacement analysis allowed determining those critical residues for the binding. Finally, in an attempt to optimise the production cost of the vaccine, we have determined that the minimal dose required for induction of protective antibodies can be as low as 0.5 μg of peptide. Also, KLH can be replaced as a carrier for a much cheaper alternative such as ovalbumine. All these findings implicate a substantial reduction in the cost of the vaccinal dose.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Pays-Bas</li>
</country>
<region><li>Communauté de Madrid</li>
</region>
</list>
<tree><country name="Pays-Bas"><noRegion><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name>
</noRegion>
<name sortKey="Boshuizen, Ronald S" sort="Boshuizen, Ronald S" uniqKey="Boshuizen R" first="Ronald S." last="Boshuizen">Ronald S. Boshuizen</name>
<name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Martinez Torrecuadrada, Jorge" sort="Martinez Torrecuadrada, Jorge" uniqKey="Martinez Torrecuadrada J" first="Jorge" last="Martinez-Torrecuadrada">Jorge Martinez-Torrecuadrada</name>
</region>
<name sortKey="Ignacio Casal, J" sort="Ignacio Casal, J" uniqKey="Ignacio Casal J" first="J." last="Ignacio Casal">J. Ignacio Casal</name>
<name sortKey="Ignacio Casal, J" sort="Ignacio Casal, J" uniqKey="Ignacio Casal J" first="J." last="Ignacio Casal">J. Ignacio Casal</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003590 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003590 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:2E63C5A7E13423A3CB64ADD4311E0C1C1F5DCF68
   |texte=   Characterisation of a protective linear B cell epitope against feline parvoviruses
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Characterisation of a protective linear B cell epitope against feline parvoviruses

Characterisation of a protective linear B cell epitope against feline parvoviruses

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri